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Mastitis Media Plates

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Eurofins manufactures and sells a full line of Mastitis Media Plates for On Farm and Vet Clinic/laboratory use in culturing for mastitis causing organisms. This includes both our Mastitis quad plates and mycoplasma plates. The Eurofins dairy mastitis quad plates for Staph, Strep and Gram Negatives (SSGN and SSGNC) are screening methods that aid the health providers and dairy producers in identifying the prevalence of these bacteria. Follow up therapy or sanitation strategies should be implemented in consultation with your veterinarian, herdsman and dairy co-op field man. Proper use of the Eurofins mastitis quad plates can aid the dairy producer in taking corrective action sooner.

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The User Guide for the quad plates contains instructions for the SSGN (Staph, Strep and Gram Negative) plate and the SSGNC (Staph, Strep, and Gram Negative Chromogenic) plate. The bacterial growth medias in both plates are identical. The only difference is in Section 2 of the SSGNC where an enzyme substrate specific for E. coli has been added. This substrate is utilized by 95% to 97% of all E. coli isolates to stain the colonies blue or purple. An exception is E. coli 0157:H7 which does not turn blue or purple, but will give the typical pink precipitate surrounding a pink colony on both plates.

Item ID Item Description

222200 Mastitis SSGN Quad Plate -Staph, Strep, Gram Negative all in one. 
Section 1 Non-selective blood agar capable of growing most bacteria. It is used as a growth control. 
Section 2 Is specific for Gram negatives such as coliforms, E. coli, and Klebsiella. 
Section 3 Is specific for Streptococcus. Section 4 Is specific for Staphylococcus. 

222210 Mastitis SSGNC Quad Plate -Staph, Strep, Gram Negative all in one. Same as item 
222200 except that E. coil colonies on the SSGNC plate will be blue or purple while E. coli colonies on the SSGN plate will be pink with a pink precipitate surrounding the colony. An exception on the SSGNC plate is E. coli 0157:H7 which does not turn blue or purple, but will give the typical pink precipitate surrounding a pink colony. 

223000 Strep ID Tri Plate- For differentiation and presumptive identification of streptococci species (Enterococcus faecalis, Streptococcus bovis, Streptococcus agalactia, Streptococcus dysgalactia, Streptococcus uberis). 

222900 Mycoplasma Plate - For cultivation and selective isolation of mycoplasma 

222100 Strep/Staph Bi Plate II - Staphylococcus media for isolation of Staph organisms including Staph aureus. Streptococcus media for isolation of Strep organisms including Strep agalactia. 

222000 General Bi Plate I - Non selective Blood Agar for Total Count and Hemolytic Reactions / selective gram negative agar. 

222400 Sensitivity Agar Plate - Agar media for sensitivity testing using the Kirby Bauer method. 

222500 Sensitivity (fastidious) Agar Plate with blood - Agar media with 5% blood added for growth of the more fastidious bacteria. 

222600 MacConkey Agar Plate: For isolation and differentiation of gram negative bacilli. 

222700 Strep Media Plate (TKT-FC): meets National Mastitis Council recommendations 

222800 Staph Media Plate (KLMB): meets National Mastitis Council recommendations 

222300 Blood Agar Plate: Tryptic soy agar with 5% sheep blood.