Enzymes can be a beneficial additive to animal feeds and in human dietary supplements. Their use as digestive aids and metabolic catalysts is becoming increasingly more common. Of particular interest are the major digestive enzymes amylases, and proteases; which aid in the digestion of starches, and proteins, respectively.
In the feed industry, Phytase is used to improve phosphorus availability in plant-based feed materials and other ingredients, while xylanase breaks down xylans – a pervasive anti-nutrient in plant based feed stuffs. Measuring the activity of these enzymes requires carefully controlled conditions.
Enzymes are relatively sensitive to environmental and manufacturing processes, including temperature, moisture and interactions with other ingredients. It is important to verify the actual enzyme activity in the final product, rather than relying on the formulated concentration. As functional bioactive molecules, enzymes may experience degradation or loss of activity during their storage. It is therefore very important that the appropriate analytical method is used. It is also a special characteristic of enzymes that their optimal working conditions (e.g. pH, temperature, metals, cofactors, and specific substrates) vary depending on the source and type of enzyme. In order to enable our laboratory to perform the appropriate assay, please provide us with information about the type and source (or manufacturer) of the enzyme when possible.
Eurofins offers the following enzyme assays:
Amylase and protease activity measurements using USP methods
Commercial phytase using methods publicized by ISO, AOAC, DSM or other manufacturers. These assays can be conducted on enzyme concentrates, premixes or finished feeds.
Xylanase using published methods. Lactase using a commercially available test kit.
Mammalian protein qualification via a FeedCheck Kit.
Proprietary assays with client supplied method or published peer-reviewed methods.