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Vitamin Testing

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Vitamins are essential nutrients for the normal growth, development and maintenance of cells, tissues, and organs. They enable the use of the chemical energy provided by food and process the proteins, carbohydrates, and fats required for respiration. Vitamins are classified as either water-soluble or fat-soluble. In humans, there are 13 vitamins: 4 fat-soluble (A, D, E and K) and 9 water-soluble (8 B vitamins and vitamin C). For the most part, vitamins are obtained via the consumption of food or dietary supplements.

Eurofins Nutrition Analysis Center provides vitamin testing on a variety of matrices including food, pet food, feed, dietary supplements, premixes, oils, etc. The lab will run the appropriate method depending on the expected level provided, which includes quantification by HPLC, GC, microbial, or fluorometric based methodologies.

Vitamin Name

Limit of Quantitation

Method for Quantification

Vitamin A (Retinol)

30 IU/100g

HPLC

Vitamin A (Beta Carotene)

30 IU/100g

HPLC

Vitamin B1 (Thiamine)

0.011 mg/100g

HPLC or Fluorometric

Vitamin B2 (Riboflavin)

0.1 mg/100g

HPLC or Fluorometric

Vitamin B3 (Niacin, Niacinamide)

0.22 mg/100g

HPLC or Microbial

Vitamin B5 (Pantothenic Acid)

0.055 mg/100g

HPLC or Microbial

Vitamin B6 (Pyridoxine)

0.01 mg/100g

HPLC

Vitamin B7 (Biotin)

0.00366 mg/100g

HPLC or Microbial

Vitamin B9 (Folic Acid)

0.0033 mg/100g

HPLC or Microbial

Vitamin B12 (Cobalamin)

0.44 μg/100g

HPLC or Microbial

Vitamin C (Ascorbic Acid)

0.44 mg/100g

HPLC or Fluorometric

Vitamin D (Total D, D2, and D3)

25 IU/100g

HPLC

Vitamin E (Tocopherols & Tocotrienols)

0.1 mg/100g

HPLC