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Steviol Glycosides Testing (JECFA)

Steviol Glycoside commercial materials (extracts) are obtained from the leaves of Stevia rebaudiana Bertoni. The 69th JECFA Chemical and Technical Assessment defines a steviol glycoside commercial material as containing at least 95% of total (seven) steviol glycosides (dried basis). The JECFA monograph defines assays to assess the identification and purity of the commercial material. The list of tests follows:

Identification

• Solubility (freely soluble in water)
• Chromatographic Identification (Stevioside and Rebaudioside A)
• pH (between 4.5 and 7.0)

Purity

• Total Ash (not more than 1%)
• Loss on Drying (not more than 6%)
• Residual Solvents (methanol and ethanol)
• Arsenic
• Lead
• Assay (HPLC seven steviol glycosides)

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