Testing for Glucosamine in Dietary Supplements, Pet Food, and Ingredients
Authored by Dr. Hong You, Director of Scientific Affairs
Glucosamine
Glucosamine is an amino sugar found in animal and human cartilage.
Clinical studies suggested that glucosamine has the potential to be used to relieve joint pain causing by Osteoarthritis and Rheumatoid Arthritis.
In supplement form, glucosamine can be harvested from shells of shellfish or animal by-products. Glucosamine is bound with proteins in crude dietary ingredients such as shrimp meal or chicken meal, making it challenging to be analyzed directly. In addition, the molecule structure of glucosamine lacks a UV-absorbing chromophore, so a derivatization step is necessary for the liquid chromatography detection.
Image from PubChem via https://pubchem.ncbi.nlm.nih.gov/compound/D-Glucosamine
Determination of Glucosamine in a dietary supplement or ingredient
Eurofins Botanical Laboratory in Brea California (formerly known as Analytical Laboratories in Anaheim, Inc.) is a market leader for Glucosamine testing for 20 years. We offer several methods for the characterization and quantification of glucosamine in dietary supplements and ingredients. The selection of the testing technique is dependent on the sample matrix, formulation, and the method used when establishing product specifications.
Our glucosamine methods have been validated and are ISO-17025 accredited.
The test codes and method performance are listed below:
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Assay Name: |
Determination of Glucosamine by UPLC |
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Scope: |
This method is suitable for high purity material, dietary supplement products, and pet food matrices. |
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Description: |
This method quantifies glucosamine using phenyl isothiocyanate (PITC) in a pre-column derivatization reaction. Samples are then separated on a reverse phase UPLC system equipped with a BEH phenyl column, and quantified against an external standard. |
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Range: |
0.1% (w/w) to 0.5% (w/w) for Pet Foods. 0.1% (w/w) to 100% (w/w) for Dietary Supplements and Pure Materials. |
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Limit of Quantitation: |
Typical LOQ is 198 µg/g (ppm) for Pet Foods. Typical LOQ is 40 µg/g (ppm) for Dietary Supplements. |
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Precision: |
1.2% RSD for High Purity Materials in Glucosamine HCL form. 2.3% RSD for High Purity Materials in Glucosamine Sulfate 2KCl form. 1.6% RSD for Dietary Supplements. 2.6% RSD for Pet Foods. |
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Minimum Sample Required: |
High Purity Materials: 5 g Dietary Supplements: 20 g or 20 Units, whichever is greater Pet Foods: 30 g |
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Method Reference: |
In-House developed method TM14438 |
