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Our objective was to compare the efficacy of commercially available pathogen screening platforms for detecting Salmonella in probiotic cultures to permit evidence-based platform selection.

This study seeks to establish the detectability of Listeria species in probiotic cultures and estimate the sensitivity of detection.

Enumeration provides the most basic required information with methodologies evolving to provide improved accuracy, precision, and turnaround times. Acoustic-assisted hydrodynamic flow cytometry incorporates the advance of using sound waves to align the particles in the fluid stream for counting, reducing the time to result.

As probiotic consumption rates continue to grow, stakeholders are demanding more information about the quantity, quality, and identity of organisms in probiotic products. Increasingly, flow cytometry is being used to address manufacturers’ and consumers’ demands, and has shown promise in rapidly providing precise quantitative and qualitative probiotic information.

Salmonella depends on an effective food safety program supported by robust Salmonella detection methods. Our objective is to explore recovery of Salmonella from a background of probiotic cultures and demonstrate and validate as effective, sensitive method for detecting Salmonella in probiotic cultures to protect public health.

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