Assessing Robustness of Salmonella and Listeria detection by Loop-mediated AMPlification (LAMP)
Validation demonstrates that methods perform “as advertised.” For major pathogen platforms, “official” validations typically cover one enrichment temperature and a narrow range of incubation times. Unfortunately, many issues—from equipment failure to human error—can disrupt the validation envelope, causing delays or loss of results. This poster—originally presented at IFT’s annual 2019 meeting—seeks to determine the robustness of LAMP pathogen assays by testing wide variations in temperature, incubation time, and other conditions, to facilitate efficient laboratory operation in “real world” conditions. This study focuses on the detection of Salmonella, Listeria spp. and L. monocytognes by LAMP using appropriate 3M MDA2 kits in cheese samples.
Authors:
Megan S. Brown and J. David Legan
Eurofins Microbiology Laboratories, Inc., Madison, WI
*Corresponding author: meganbrown@eurofinsus.com