Case Study: Unlocking Clarity in Green Tea Extract Analysis
Client Challenge
A client faced complications when analyzing a green tea extract sample using a Eurofins internal testing method. The sample was compared to a crude raw botanical reference material, and while some bands in the sample aligned with the reference, others were missing. As a result, the analysis was deemed inconclusive, leaving the client unable to confirm the extract’s quality and consistency. The client reached out to the team of botanical experts at the Eurofins Botanical Laboratory in Brea, CA for support on solving this challenge.
Eurofins’ Investigation Approach
Eurofins collaborated closely with the client to uncover the root cause of the inconclusive results.
- Problem Analysis: Through in-depth discussions about the material and the methodology used in the initial analysis, Eurofins identified a potential issue: the green tea extract may have been processed in a way that altered the HPTLC fingerprint, eliminating certain bands from the profile.
- Method Recommendation: Drawing on their expertise, Eurofins suggested switching to a compendial method for green tea extract analysis. This method relies on specific marker compounds—4 catechins and caffeine—as acceptance criteria for evaluation, offering a more precise and reliable assessment.
Results
The compendial method provided the clarity the client was seeking:
- The green tea extract sample met all acceptance criteria, containing the required 5 marker compounds (4 catechins + caffeine).
- This outcome underscored the challenges posed by the variability in natural products and highlighted the importance of tailored testing methodologies. As each testing approach is designed for a specific scope, troubleshooting is often necessary to align methods with the characteristics of the product.
The Eurofins Solution
Eurofins demonstrated the critical role of partnering with a trusted third-party laboratory to resolve complex analytical challenges. By leveraging their deep expertise and advanced testing capabilities, Eurofins provided the client with:
- Comprehensive data to support accurate botanical identification.
- Actionable insights and best practices for optimizing method selection.
- Confidence in the quality and compliance of their product.
This partnership exemplifies Eurofins’ commitment to empowering stakeholders in the natural products industry, helping them navigate technical hurdles, maximize quality, and enhance profitability.
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