Comparison of the BACGene Listeria spp. Real Time PCR and BAX® System 24E Genus Listeria PCR Methods for the Detection of Genus Listeria in Food and Environmental Samples from Two Dairy Production Facilities
A large dairy product manufacturer expressed interest in an alternate PCR method for the detection of Genus Listeria in their environmental and product testing program due to recurring false-positive detections and high levels of environmental Genus Listeria positives in the recent past. Our objective was to compare the performance of two PCR based methods for Genus Listeria detection. BAX© System 24E and BACGene Listeria species were used to test 244 environmental samples and 33 product samples in the same fashion as the facilities’ existing ongoing and monitoring programs—ensuring, study results would represent real-world conditions including, collection, shipping and analysis.
Authors:
Daniel R. DeMarco, Colin O'Malley, Jennifer Willig, Douglas Marshall, Roger Hooi and Anita Gerung