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This study seeks to establish the detectability of Listeria species in probiotic cultures and estimate the sensitivity of detection.
Pathogen detection in foods and environmental samples is a critical element in a food management system. This study set out to compare the robustness of Loop Mediated Amplification (LAMP) with that of PCR as illustrated by the detection threshold of Listeria and Salmonella spiked into culture broths previously incubated with various spices.
Enumeration provides the most basic required information with methodologies evolving to provide improved accuracy, precision, and turnaround times. Acoustic-assisted hydrodynamic flow cytometry incorporates the advance of using sound waves to align the particles in the fluid stream for counting, reducing the time to result.
Probiotic Enumeration by Flow Cytometry: Method Scope Extension of ISO 19344 - Benkowski and Schoeni
As probiotic consumption rates continue to grow, stakeholders are demanding more information about the quantity, quality, and identity of organisms in probiotic products. Increasingly, flow cytometry is being used to address manufacturers’ and consumers’ demands, and has shown promise in rapidly providing precise quantitative and qualitative probiotic information.
For microbiology both quantity and quality matter, and microbiological methods are classified as being either quantitative or qualitative. Which category they fall into is based on what type of data is actually collected and reported.
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